Summary
SW982 cells are characterized by expression of inflammatory cytokine and matrix metalloproteinase (MMP) genes and by their response to dexamethasone at different cell densities. They express genes encoding interleukin (IL)-1β; IL-6; transforming growth factor-β; intercellular adhesion molecule-1; cycloxygenase (COX)-2; and MMPs, including MMP-1, MMP-2, MMP-13, and MT1-MMP; tissue inhibitor of metalloproteinase-2; and a disintegrin and metalloproteinase with thrombospondin motifs-4. Expression of all the genes examined was induced with 2 ng/ml IL-1β at low cell density. The cells, however, failed to express tumor necrosis factor-α, COX-1, and MMP-9, regardless of the presence of IL-1β. Dexamethasone significantly reduced IL-1β, IL-6, COX-2, and MMP-1 expression at high cell density. The results suggest that SW982 cells are a useful tool for studying the expression of inflammatory cytokine or MMP genes.
Similar content being viewed by others
References
Anphan, N.; DiDonato, J. A.; Rossette, C.; Helmberg, A.; Karin, M. Immunosupression by glucocorticoids: inhibition of NFκB activity through induction of IκB synthesis. Science 270:286–290; 1995.
Chomczynski, P.; Sacchi, N. Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156–159; 1987.
Lipsky, P. E. Rheumatoid arthritis. In: Issel bacher, K. J., ed. Harrisons principles of internal medicine. New York: McGraw Hill; 1994: 1648–1655.
Martel-Pelletier, J.; Cloutier, J.-M.; Pelletier, J.-P. In vivo effects of antirheumatic drugs on neutral collagenolytic proteases in human rheumatoid arthritis cartilage and synovium. J. Rheumatol. 15:1198–1204; 1988.
Sakane, T.; Suzuki, N.; Hirose, Y., et al. Mechanisms of KE298, 2-acetylthiomethyl-3-(4-methylbenzoyl) propionic acid, to suppress abnormal synovial cell functions in patients with rheumatoid arthrithis. J. Rheumatol. 24:2213–2220; 1997.
Scheinman, R. I.; Gualberto, A.; Jewell, C. M.; Cidlowski, J. A.; Baldwin, A. S. J. Characterization of mechanisms involved in transrepression of NFκB by activated glucocorcoid receptor. Mol. Cell. Biol. 15:943–958; 1995.
Seki, M.; Uzuki, M.; Ohmoto, H.; Yoshino, K.; Maeda, S.; Kokubun, S.; Sakurai, M.; Sawai, T. Matrix metalloproteinase (MMP-9) in patients with rheumatoid arthritis. Jpn. J. Rheumatol. 35:972–801; 1995.
Tsuji, F.; Oki, K.; Senda, T.; Horiuchi, M.; Mita, S. Effects of mitogen-activated protein kinase inhibitors or phosphodiesterase inhibitors on interleukin-1-induced cytokines production in synovium-derived cells. Immunol. Lett. 68:275–279; 1999.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yamazaki, T., Yokoyama, T., Akatsu, H. et al. Phenotypic characterization of a human synovial sarcoma cell line, SW982, and its response to dexamethasone. In Vitro Cell.Dev.Biol.-Animal 39, 337–339 (2003). https://doi.org/10.1290/1543-706X(2003)039<0337:PCOAHS>2.0.CO;2
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1290/1543-706X(2003)039<0337:PCOAHS>2.0.CO;2